| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1221 | Massilioclostridium coli | TGCGGATGGTACGAACAGC | TCGCCATCTTTCCAGAGACG | 60.45 | 59.83 | 207 | 50 |
100.00%
|
100.00%
|
| Primer 1222 | Massilioclostridium coli | AAAACGCACCGGAGCTCA | GCAGGAAGTTCGAGGCTCT | 59.89 | 59.41 | 195 | 50 |
100.00%
|
100.00%
|
| Primer 1223 | Massilioclostridium coli | AAACCGCAAAACGCACCG | GCAGGAAGTTCGAGGCTCT | 60.28 | 59.41 | 202 | 50 |
100.00%
|
100.00%
|
| Primer 1224 | Massilioclostridium coli | TGTGGTGTGTGCCCATGA | TCTACCCCTGAGTAAGCGGA | 59.08 | 59.37 | 207 | 50 |
100.00%
|
100.00%
|
| Primer 1225 | Massilioclostridium coli | TGCCTGCCATTTAGGGACG | TCCGCACCTTTTCCAGCA | 60.08 | 59.49 | 225 | 50 |
100.00%
|
100.00%
|
| Primer 1226 | Massilioclostridium coli | TGTTACCGTTGGGGATGGC | GGCCAATCCCACCACTTGA | 60.00 | 59.92 | 162 | 50 |
100.00%
|
100.00%
|
| Primer 1227 | Massilioclostridium coli | AACATGGCTGGGCAGTTGA | GGCGTTGGAATCGTTGCA | 59.85 | 59.05 | 221 | 50 |
100.00%
|
100.00%
|
| Primer 1228 | Massilioclostridium coli | ACAGCTCAAACTGACGCGA | ACCAGTTCGCTGCCAACT | 59.93 | 59.49 | 235 | 50 |
100.00%
|
100.00%
|
| Primer 1229 | Massilioclostridium coli | CGAGCCGAATATCCCGTCA | CCACGGGATACAACCTGCA | 59.34 | 59.70 | 212 | 50 |
100.00%
|
100.00%
|
| Primer 1230 | Massilioclostridium coli | CGAGCCGAATATCCCGTCA | ACCACGGGATACAACCTGC | 59.34 | 59.70 | 213 | 50 |
100.00%
|
100.00%
|